Describe the hilic system and prevailing theories on retention mechanisms. As problem solvers, analysts search for ways to optimize their experiments and quickly collect data. It is this moderate back pressure of halo columns that also permits them to be used with most conventional hplc equipment for uhplc performance. Now, halo pentahilic columns join halo c18, c8, rpamide, escn, phenylhexyl, pfp, peptide esc18 and the standard silica hilic phase to offer a wide range of powerful selectivity choices to accomplish the most demanding separations. Chromatography hilic is an increasingly popular technique offering complementary selectivity to reversed phase.
The halo column is based on the fusedcore technology which allows faster flow rates. Now halo pentahilic columns join halo c18, c8, rpamide, halo escn, phenylhexyl, pfp, peptide esc18 and the standard silica hilic. Hplc columns hplc column selectivity measurements of more. The amide hilic bonded phases provide strong hydrogen bonding interaction between the stationary phase and the analytes, resulting in stronger retention compared to other hilic phases. You will be logged off in seconds due to inactivity. Agilent zorbax rapid resolution high definition hilic plus. Stability and performance of cyano bonded phase hplc columns.
Nucleosides, glycosides and other sugarrelated compounds are ideal for halo hilic and penta hilic. Halo 2 uhplc columns have all of the advantages of sub2 m noncore particle columns and will deliver 300,000 plates per meter e. Provide some tips and cautions as they related to method development in hilic mode aim. With the ability to retain highly polar and hydrophilic compounds, thermo scientific syncronis hilic hplc columns have been developed to aid the analysis of compounds that are traditionally difficult to retain using. Dependable, ultrapure silicabased hplc columns that offer an extensive variety of selectivities which are scalable from microbore to preparative and purification scale solutions. Eksigent chiplc columns are a unique line of microfluidic chip based columns and traps in 75. Mobile phase should be filtered and degassed before use flush the solvent to be used through the system before installing the column connect the column taking care to match the arrow to the direction of flow. Separation of cephalosporins on halo pentahilic and halo hilic. Due to the highly polar mobile phases used in hilic, both acidic and basic compounds often exhibit highly symmetrical peak shapes, often superior to those obtained in reversedphase. Separation of cephalosporins on halo pentahilic and. New halo fusedcore pentahilic uhplc columns for enhanced.
Compared to standard hplc columns with totally porous 5 m particles, the halo5 columns feature. So take off you lab coat, relax and let our web tool do the work. Stability and performance of cyano bonded phase hplc columns for reversedphase, normalphase and hilic applications richard a. For historical reasons, it has been reported that hilic is a variant of normal phase liquid chromatography, but the separation mechanism used in hilic is more complicated than that in nplc. Cosmosil application has more than 7,000 applications using cosmosil columns. Discuss various hilic stationary phases and the interactions they exhibit 3.
What are the main differences between hilic columns and a. These columns are optimized for ultrafast hplc separations with more resolving power for small molecules. Hplc columns should be used in wellventilated environments to minimize concentration of solvent fumes. The typical particle size of traditional hplc columns is 5m, but in the past, 3. The fast separation is achieved by smaller size particles packed into the column which reduces the diffusion of solutes into and out of the stationary phase and therefore reduces peak broadening. Impurity bonusrp alkyl c8 separation of small molecule anorectics on zorbax bonusrp and traditional. All solvents used in the hplc columns should be of hplc grade or ms grade quality. Quickly and easily reversed hplc column develop methods based on compound type, usp methods, application, or desired column phase. Totally porous phase length 805100 chromxp c18, 3 m 120 a 5 cm 80510014 chromxp c18, 3 m 120 a 10 cm. Halo columns are of c8, c18, rp amide, and hilic types with 2. Now halo columns packed with a pentafl uorophenyl phase pfp join halo c18, c8, rpamide, phenylhexyl and hilic phases to offer a wide range of powerful selectivity choices to accomplish the most demanding separations. From a fast separation of a few compounds to a more complex natural product sample containing hundreds of analytes, halo. The cogent typec silica columns perform similarly to hilic columns as far as polar compound elution order is concerned when using higher than 70% organic composition of the mobile phase and both columns perform separations that are based on variations of normal phase chromatography with one being called hilic and the other called.
Wide ph stability for long column lifetime and method flexibility. Phase comparison using hilic mode pittcon 2017 rah when c18 columns dont work nucleosides and nucleobases are often used as void markers for c18 columns, but they can be easily retained by halo penta hilic. The halo5 column is based on the same fusedcore particle technology as the halo column with 2. Halo pfp is particularly well suited for the separation of. Agilent zorbax hilic plus hplc columns are nonbonded silica columns made with. The appropriate guard cartridge for a specific halo column id is listed under the accessories tab of the specific column. Lc columns and accessories columns for analytical lc hypersil gold competitor 2 competitor 1 signal 300 200 100 the improved peak symmetry provides additional peak height to increase sensitivity of analysis of trace components. Halo columns are hplc columns for uhplc at normal pressure. These columns are well suited for separating a variety of hydrophilic molecules, including carbohydrates and peptides.
Halo fusedcore columns take your uhplc and hplc methods to the next level of speed and resolution. This results in an increased seperaton power so that the halo column may either be used for faster analysis or an improved separation. Uhplc separations with either uhplc or conventional hplc equipment. Being the most common principle hplc uhplc separation. Agilent zorbax hilic plus hplc columns are nonbonded silica columns made with zorbax eclipse plus silica highpurity, type b silica, specially treated for good peak shape and optimized for hydrophilic interaction chromatography hilic separations. Hilic is typically used for the retention and separation of small, polar analytes. Halo fusedcore particle technology facilitates ultrafast, high resolution mechanism of separation uhplc separations with either uhplc or conventional hplc equipment. In addition, sample loading effects often are more favourable for hilic. Uhplc and hplc columns, this process is made simpler and less problematic.
Required for all guard cartridges is the halo guard column holder part number 94900001 see macmod accessories page. Cosmosil application provides you with sample analysis conditions with widely used ods columns and other specialty columns. Users of hplc columns should be aware of the toxicity or flammability of the mobile phases chosen for use with the columns. Figure 1 halo hilic qc chromatogram20 0 20 40 60 80 100 120. Agilent zorbax rapid resolution high definition rrhd hilic plus threaded columns are specially designed for use with ultrahigh performance liquid chromatographs uhplcs such as the agilent 1290 infinity lc and can be used up to an operating pressure of 1200 bar. Precautions should be taken to avoid contact and leaks.